![]() Three bottles go to microbiology, one to virology, one to biochemistry. Usually 5-10 drops for each sample are sufficient. Collect the fluid in five numbered bottles (In general, the CSF pressure increases with body mass index (BMI) and decreases with age after the sixth decade. When the meniscus settles, gently tap the tube if there is no movement then read the pressure in cm H 2O from the scale. Connect tubing from the manometer to the needle cuff and hold the gauge upright. Measure CSF pressure with the manometer.Withdraw the stylet and wait for CSF to appear at the needle cuff.Feel resistance from the spinal ligaments and then the dura, and feel 'give' as the needle enters the subarachnoid space. Make sure that you are in the midline between the iliac crests and in the interspinous space.After one minute, insert a 22G spinal needle with stylet in place, horizontally through the mark, aiming towards the umbilicus with the needle bevel facing upwards.ALWAYS aspirate before injecting lidocaine, to avoid intravascular injection. Let a minute pass, then infiltrate lidocaine into the interspinous area in bigger patients use a longer, larger-gauge needle if required. Use a small-gauge needle to minimise pain. Anaesthetise the skin with 1% lidocaine.If you are using a fenestrated sterile adhesive drape, the sterile field can be isolated at this point. Sterilise the area with iodine-based antiseptic unless the patient is allergic.Extra-long needles are occasionally required for very large adults. Spinal needles are available in varying sizes for different ages - from 30 mm-90 mm usually.Open your sterile LP pack and make sure your needle, sample bottles (preferably labelled '1, 2, 3' in the order you will use to collect samples) and manometer are set up and immediately to hand.From the pack, place sterile towels beneath the patient and over their side, to isolate your sterile field. From this point on you must observe strict aseptic technique. Wash your hands thoroughly and put on a mask and sterile gloves.Mark the intervertebral space L4/L5 or 元/L4 (at the same height as the iliac crest) with a gentle skin indentation using a thumbnail or an object like a pen top.The procedure may be radiologically guided, if necessary. Communicating hydrocephalus (also known as normal pressure hydrocephalus).ī staff, "Blausen gallery 2014", Wikiversity Journal of Medicine, via Wikimedia Commonsīy staff, "Blausen gallery 2014", Wikiversity Journal of Medicine, via Wikimedia CommonsĪ very useful technique in competent adults is to position the patient so that they are sitting up leaning over a table or pillow, as the anatomy is less distorted.Neuromodulation in spasticity and dystonias.This will reflect changes in chemotherapy and include intrathecal chemotherapy and acute oncology services. National guidelines on safe administration of intrathecal chemotherapy were archived in 2013, pending updated guidance. To administer medications via the CSF - intrathecal therapy:. ![]() In cases such as confusional states, meningeal malignancies, demyelinating disorders, CNS vasculitis, multiple sclerosis.Within the first 6-12 hours the differentiation between genuine subarachnoid blood and traumatic admixture of blood may be difficult.LP must be performed in a case of clinically suspected intracranial bleed or SAH if CT or MRI does not confirm the diagnosis.Suspected intracranial bleeding and subarachnoid haemorrhage (SAH):.It also allows identification of uncommon pathogens and viruses by polymerase chain reaction (PCR).Initial Gram staining shows organism in 69-93% of cases of pneumococcal meningitis, and 30-89% of cases of meningococcal meningitis in adults and children.Acellular CSF is rare, except in patients with tuberculous meningitis.Polymorphonuclear cells may predominate in cases of non-meningococcal meningitis.In immunocompromised patients, CSF white cell counts are often low.More than 90% of cases of acute bacterial meningitis present with a CSF white cell count of more than 100 cells per μL.Cerebrospinal fluid (CSF) culture is the gold standard in the diagnosis of bacterial meningitis.Lumbar puncture (LP) can confirm or exclude meningitis.Serious complications are rare, and correct technique will minimise diagnostic error and maximise patient comfort. Evaluation of an acute headache and investigation of inflammatory or infectious disease of the nervous system are the most common indications. Diagnostic lumbar puncture is one of the most commonly performed invasive tests in hospital clinical medicine.
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